Detection of extended-spectrum β-lactamase-producing Escherichia coli genes isolated from cat rectal swabs at Surabaya Veterinary Hospital, Indonesia

Background and Aim: Escherichia coli causes a bacterial illness that frequently affects cats. Diseases caused by E. are treated using antibiotics. Because of their proximity to humans, cats possess an extremely high risk contracting antibiotic resistance genes when owners touch cat feces containing harbor genes. This study was conducted identify multidrug-resistant extended-spectrum β-lactamase (ESBL)-producing from rectal swabs collected at Surabaya City Veterinary Hospital determine sensitivity. Materials Methods: Samples were cultured in Brilliant Green Bile Lactose Broth medium then streaked on eosin methylene blue agar for isolation, whereas Gram-staining IMViC tests confirm the identification results. The Kirby–Bauer diffusion test used sensitivity, double-disk synergy ESBL-producing bacteria. Molecular detection TEM CTX-M performed polymerase chain reaction. Results: Based morphological culture, Gram-staining, biochemical testing, results sample inspection showed 100 swab samples isolated, 71 (71%) positive coli. Furthermore, 23 isolates (32.39%) demonstrated highest ampicillin. Four confirmed be multidurg-resistant strains. examination revealed three harbored CTX-M. Conclusion: In conclusion, pet must educated use antibiotics improve knowledge about risks resistance. Keywords: cat, coli, β-lactamase, multidrug resistance, public health.